Robust Analysis of High Throughput Screening (HTS) Assay Data
نویسندگان
چکیده
منابع مشابه
HTS-Corrector: software for the statistical analysis and correction of experimental high-throughput screening data
MOTIVATION High-throughput screening (HTS) plays a central role in modern drug discovery, allowing for testing of >100,000 compounds per screen. The aim of our work was to develop and implement methods for minimizing the impact of systematic error in the analysis of HTS data. To the best of our knowledge, two new data correction methods included in HTS-Corrector are not available in any existin...
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High-Throughput Screening (HTS) data in its entirety is valuable raw material for the drug-discovery process. It provides the most complete information about the biological activity of a company's compounds. However, its quantity, complexity and heterogeneity require novel, sophisticated approaches in data analysis. At GeneData, we are developing methods for large-scale, synoptical mining of sc...
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With the advances in biotechnology, identification of new therapeutic targets, and better un‐ derstanding of human diseases, pharmaceutical companies and academic institutions have accelerated their efforts in drug discovery. The pipeline to obtain therapeutics often involves target identification and validation, lead discovery and optimization, pre-clinical animal studies, and eventually clini...
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SUMMARY We report on the development of the high-throughput screening (HTS) Navigator software to analyze and visualize the results of HTS of chemical libraries. The HTS Navigator processes output files from different plate readers' formats, computes the overall HTS matrix, automatically detects hits and has different types of baseline navigation and correction features. The software incorporat...
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Our laboratory has previously developed a qRT-PCR assay to assess drug efficacy on the growth of Cryptosporidium parvum in vitro by detecting the levels of parasite 18S rRNA. This approach displayed up to four orders of magnitude of linear dynamic range and was much less labor-intensive than the traditional microscopic methods. However, conventional qRT-PCR protocol is not very amendable to hig...
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ژورنال
عنوان ژورنال: Technometrics
سال: 2013
ISSN: 0040-1706,1537-2723
DOI: 10.1080/00401706.2012.749166